Liquid Biopsy and Non-Invasive Diagnostics

Liquid biopsy and other non-invasive diagnostic techniques are revolutionizing the landscape of breast cancer detection, prognosis, and treatment monitoring. Unlike traditional biopsies that require surgical or tissue-based sampling, liquid biopsies analyze circulating biomarkers—such as cell-free DNA (cfDNA), circulating tumor cells (CTCs), and exosomes—from a simple blood sample. Liquid biopsies offer a minimally invasive alternative to tissue biopsies, allowing for frequent sampling over time and providing a dynamic view of tumor evolution. They are particularly useful in cases where tumor tissue is difficult to access or when serial monitoring is required to assess treatment response or detect recurrence. A major highlight will be the role of ctDNA in early cancer detection, prognosis, and treatment monitoring. Studies have demonstrated that specific mutations, methylation patterns, and tumor-specific alterations can be identified in ctDNA with high sensitivity. Although less abundant than ctDNA, CTCs provide unique insights into the biology of metastatic disease and can be cultured or analyzed for gene expression and protein markers, aiding in real-time phenotyping of the tumor. 

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